Cloned (Comment) | Organism |
---|---|
expression of nSMase in Bacillus brevis 47 | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
H151A | 0.6% of wild-type nSMase activity | Bacillus cereus |
H151D | no nSMase activity | Bacillus cereus |
H151E | no nSMase activity | Bacillus cereus |
H151F | no nSMase activity | Bacillus cereus |
H151K | no nSMase activity | Bacillus cereus |
H151N | no nSMase activity | Bacillus cereus |
H151Q | 8.3% of wild-type nSMase activity | Bacillus cereus |
H151R | no nSMase activity | Bacillus cereus |
H151Y | no nSMase activity | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | extracellular soluble toxin that induces hemolysis by acting on sphingomyelin-rich erythrocytes of mammals such as ruminants | Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ | Bacillus cereus | |
Mg2+ | required for activity, Glu53 act as a ligand for the essential Mg2+ | Bacillus cereus | |
Mn2+ | can replace Mg2+ | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Bacillus cereus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant nSMase | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O | - |
Bacillus cereus | 2-hexadecanoylamino-4-nitrophenol + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Bacillus cereus | N-acylsphingosine + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase | - |
Bacillus cereus |
nSMase | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
sphingomyelin | 37°C, pH 7.5 | Bacillus cereus |