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Literature summary for 3.1.4.12 extracted from

  • Mizutani, Y.; Tamiya-Koizumi, K.; Irie, F.; Hirabayashi, Y.; Miwa, M.; Yoshida, S.
    Cloning and expression of rat neutral sphingomyelinase: enzymological characterization and identification of essential histidine residues (2000), Biochim. Biophys. Acta, 1485, 236-246.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol approx. 10fold activation at 3-4 mM Rattus norvegicus
Triton X-100 required for full activity Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H136A no activity Rattus norvegicus
H151A no activity Rattus norvegicus
H151Y no activity Rattus norvegicus
H272A no activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
sphingomyelin 37°C, pH 7.4 Rattus norvegicus
0.048
-
1-alkyl-lyso-platelet activating factor 37°C, pH 7.4 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 1 M, 3fold activation Rattus norvegicus
Mg2+ absolutely required for activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47600
-
x * 47600, deduced from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Rattus norvegicus
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant nSMase Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alkyl-lyso-platelet activating factor + H2O
-
Rattus norvegicus 1-alkylglycerol + choline phosphate
-
?
sphingomyelin + H2O
-
Rattus norvegicus N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
? x * 47600, deduced from nucleotide sequence Rattus norvegicus

Synonyms

Synonyms Comment Organism
neutral sphingomyelinase
-
Rattus norvegicus
nSMase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.4 approx. 25% of maximal actiity at pH 6.0, approx. 75% of maximal activity at pH 8.0 Rattus norvegicus