Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | approx. 10fold activation at 3-4 mM | Rattus norvegicus | |
Triton X-100 | required for full activity | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H136A | no activity | Rattus norvegicus |
H151A | no activity | Rattus norvegicus |
H151Y | no activity | Rattus norvegicus |
H272A | no activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
sphingomyelin | 37°C, pH 7.4 | Rattus norvegicus | |
0.048 | - |
1-alkyl-lyso-platelet activating factor | 37°C, pH 7.4 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 1 M, 3fold activation | Rattus norvegicus | |
Mg2+ | absolutely required for activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47600 | - |
x * 47600, deduced from nucleotide sequence | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Rattus norvegicus | - |
N-acylsphingosine + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant nSMase | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-alkyl-lyso-platelet activating factor + H2O | - |
Rattus norvegicus | 1-alkylglycerol + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47600, deduced from nucleotide sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
neutral sphingomyelinase | - |
Rattus norvegicus |
nSMase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.4 | approx. 25% of maximal actiity at pH 6.0, approx. 75% of maximal activity at pH 8.0 | Rattus norvegicus |