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Literature summary for 3.1.4.12 extracted from

  • Chan, E.C.; Chang, C.C.; Li, Y.S.; Chang, C.A.; Chiou, C.C.; Wu, T.Z.
    Purification and characterization of neutral sphingomyelinase from Helicobacter pylori (2000), Biochemistry, 39, 4838-4845.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 20 mM, complete inhibition Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, maximal activity at 8-16 mM, Km at 3 mM Helicobacter pylori
Mn2+ can replace Mg2+, Km at 2.5 mM Helicobacter pylori
Zn2+ slight activation at 4 mM, Km at 2 mM Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylethanolamine + H2O Helicobacter pylori low activity diacylglycerol + ethanolamine phosphate
-
?
sphingomyelin + H2O Helicobacter pylori
-
N-acylsphingosine + choline phosphate
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acid glycine, ammonium sulfate, CM Sepharose, Mono Q, Sephadex G-75 Helicobacter pylori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
-
Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylethanolamine + H2O low activity Helicobacter pylori diacylglycerol + ethanolamine phosphate
-
?
sphingomyelin + H2O
-
Helicobacter pylori N-acylsphingosine + choline phosphate
-
?

Synonyms

Synonyms Comment Organism
N-SMase
-
Helicobacter pylori
neutral sphingomyelinase
-
Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 approx. 10% of maximal activity at pH 6.0 and pH 8.5, respectively Helicobacter pylori

pI Value

Organism Comment pI Value Maximum pI Value
Helicobacter pylori isoelectric focusing
-
7.15