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Literature summary for 3.1.4.12 extracted from

  • Fensome, A.C.; Rodrigues-Lima, F.; Josephs, M.; Paterson, H.F.; Katan, M.
    A neutral magnesium-dependent sphingomyelinase isoform associated with intracellular membranes and reversibly inhibited by reactive oxygen species (2000), J. Biol. Chem., 275, 1128-1136.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Mus musculus
dithiothreitol activation in a dose dependent manner Mus musculus
reduced glutathione activation in a dose dependent manner Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Mus musculus
glutathione then oxidized Mus musculus
Ni2+
-
Mus musculus
Zn2+
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00059
-
sphingomyelin
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Mus musculus 5783
-
membrane
-
Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Mus musculus
Mg2+ dependent Mus musculus
Mn2+
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE and Western blotting analysis Mus musculus
90000 100000 gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10fold Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
liver
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Mus musculus N-acylsphingosine + choline phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE and Western blotting analysis Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
acidic sphingomyelinase Mus musculus
7
-
neutral sphingomyelinase Mus musculus