Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethylammonium bromide | low concentrations | Rattus norvegicus | |
Detergents | - |
Rattus norvegicus | |
Triton X-100 | - |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ceramide | 23% inhibition | Rattus norvegicus | |
cetyltrimethylammonium bromide | - |
Rattus norvegicus | |
phosphorylcholine | 23% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | 0.9 | sphingomyelin | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus norvegicus | 5764 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, 21fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-18°C, 0.25 M sucrose, or 20% glycerol, or 33% (NH4)2SO4 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | erythro-isomers of DL-trans-2-N-palmitoyl-1-O-phosphorylcholinesphingosine or dihydrosphingosine hydrolyzed faster than the threo-isomer | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
inactive after 10 min | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | depending on buffer | Rattus norvegicus |