Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | 20% activation of enzyme A and B | Homo sapiens | |
Triton X-100 | activation of enzyme A and B | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
sphingomyelin | enzyme B | Homo sapiens | |
0.3 | - |
sphingomyelin | enzyme A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5fold activation of enzyme A and B at pH 7.5 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration, enzyme B | Homo sapiens |
150000 | - |
enzyme A, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme A 800fold pure, enzyme B 2500fold pure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
10 mM Tris-HCl, pH 7.2, 0. 1% Triton X-100, 10 mM 2-mercaptoethanol, protein 0.1 mg/ml, more than 2 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
up to | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
enzyme A and B | Homo sapiens |