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Literature summary for 3.1.4.12 extracted from

  • Pentchev, P.G.; Brady, R.O.; Gal, A.E.; Hibbert, S.R.
    The isolation and characterization of sphingomyelinase from human placental tissue (1977), Biochim. Biophys. Acta, 488, 312-321.
    View publication on PubMed

General Stability

General Stability Organism
albumin stabilizes Homo sapiens
glycerol, stabilization Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular extraction maximized with cutsum Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28300
-
1 * 36800 + 1 * 28300, SDS-PAGE Homo sapiens
36800
-
1 * 36800 + 1 * 28300, SDS-PAGE Homo sapiens
290000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10000fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Homo sapiens
-
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
different assay conditions, substrates etc. Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 25 mM citrate buffer, pH 5.9, 2 mg/ml cutsum, 50% glycerol, 5 mM EDTA, 1 mM dithiothreitol, more than 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O
-
Homo sapiens 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate
-
?
sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?
sphingomyelin + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 36800 + 1 * 28300, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Homo sapiens
5 5.5 hexadecanoyl-p-nitrophenyl phosphorylcholine Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5 natural substrate Homo sapiens