Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | lysosomes | Rattus norvegicus | |
AMP | lysosomes | Rattus norvegicus | |
Hg2+ | plasma membrane and microsomes | Rattus norvegicus | |
Mg2+ | lysosomal enzyme, inhibition at below pH 4.8 | Rattus norvegicus | |
n-butanol | plasma membrane | Rattus norvegicus | |
p-chloromercuribenzoate | plasma membrane and microsomes | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
sphingomyelin | microsomes | Rattus norvegicus | |
1.2 | - |
sphingomyelin | lysosomes, at 0.5 mM sphingomyelin | Rattus norvegicus | |
13 | - |
sphingomyelin | lysosomes, at 2 mM sphingomyelin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
lysosome | - |
Rattus norvegicus | 5764 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | lysosomal enzyme, stimulation at pH 5-7.5 | Rattus norvegicus | |
Co2+ | lysosomal enzyme, stimulation at pH 5-7.5 | Rattus norvegicus | |
Mg2+ | lysosomal enzyme, stimulation at pH 5-7.5 | Rattus norvegicus | |
Mg2+ | activation of plasma membrane and microsomal enzyme | Rattus norvegicus | |
Mn2+ | lysosomal enzyme, stimulation at pH 5-7.5 | Rattus norvegicus | |
Mn2+ | activation of plasma membrane and microsomal enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
different assay conditions, substrates etc. | Rattus norvegicus |
additional information | - |
microsomes, lysosomes and plasma membrane | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
plasma membrane and microsomes, 10 min, 15% activity, lysosomes, 10 min, 78% activity | Rattus norvegicus |
70 | - |
denaturation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | 4.8 | in lysosomes | Rattus norvegicus |
7 | 7.2 | plasma membranes | Rattus norvegicus |
7.2 | - |
microsomes | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | plasma membranes | Rattus norvegicus |
5 | 7.5 | lysosomal enzyme stimulated by different divalent cations | Rattus norvegicus |