Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.12 extracted from

  • Jones, C.S.; Shankaran, P.; Callahan, J.W.
    Purification of sphingomyelinase to apparent homogeneity by using hydrophobic chromatography (1981), Biochem. J., 195, 373-382.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
sphingomyelin
-
Homo sapiens
14.5
-
bis-p-nitrophenyl phosphate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30700
-
1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens
47500
-
1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens
89100
-
1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10000-140000fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
different assay conditions, substrates etc. Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 0.5% Triton X-100, more than 4 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(4-methylumbelliferyl)-phosphate + H2O
-
Homo sapiens ?
-
?
bis-p-nitrophenyl phosphate + H2O
-
Homo sapiens ?
-
?
sphingomyelin + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
trimer 1 * 89100 + 1 * 47500 + 1 * 30700, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
Homo sapiens
4.8 5 bis-4-methylumbelliferyl phosphate and bis-p-nitrophenyl phosphate Homo sapiens