Application | Comment | Organism |
---|---|---|
food industry | the enzyme is used in industrial soybean oil degumming | Lysinibacillus sphaericus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21AI cells | Lysinibacillus sphaericus |
General Stability | Organism |
---|---|
the presence of Ca2+ increases the thermal and chemical stability of the protein both in aqueous media and in oil | Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the presence of Ca2+ increases the thermal and chemical stability of the protein both in aqueous media and in oil | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | Lysinibacillus sphaericus | - |
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Lysinibacillus sphaericus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
butyl-fluorescein myo-inositol phosphate + H2O | - |
Lysinibacillus sphaericus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 451600, His-tagged enzyme, calculated from amino acid sequence | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol phospholipase C | - |
Lysinibacillus sphaericus |
PIPLC | - |
Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | without Ca2+, the full-length protein retains 85% of its initial activity after treatment at 50°C. The enzyme is almost completely inactivated after incubation at 60°C .The enzyme retains more than 99% of its initial activity at 50°C and over 40% at 60°C in the presence of Ca2+. | Lysinibacillus sphaericus |