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Literature summary for 3.1.4.11 extracted from

  • Sanchez-Cach, L.A.; Ortiz-Garcia, M.M.; Minero-Garcia, Y.; Munoz-Sanchez, J.A.; Hernandez-Sotomayor, S.T.; Suarez-Solis, V.M.; De Los Santos-Briones, C.
    Isolation of cDNA encoding the catalytic site of phosphatidylinositol-specific phospholipase C from Coffea arabica L.: Recombinant expression and peptide purification (2008), Plant Signal. Behav., 3, 913-916.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment) Organism
into the pET11d vector, expressed as a histidine-tagged protein in Escherichia coli BL21 cells Coffea arabica

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Catharanthus roseus 16020
-
membrane
-
 Capsicum chinense 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, SDS-PAGE or Western blot analysis Coffea arabica
30000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense
32000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense
34700
-
 sequence analysis Coffea arabica
44000
-
 x * 44000, Western blot analysis Catharanthus roseus
44000
-
 x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense

Organism

Organism UniProt Comment Textmining
Capsicum chinense
-
-
-
Catharanthus roseus
-
-
-
Coffea arabica B2LSE7
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA chromatography Coffea arabica

Source Tissue

Source Tissue Comment Organism Textmining
additional information L2 cell line and aluminum-tolerant LAMt cell line Coffea arabica
-
root
-
 Coffea arabica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylinositol 4,5-bisphosphate + H2O
-
 Coffea arabica D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE or Western blot analysis Coffea arabica
? x * 44000, Western blot analysis Catharanthus roseus
? x * 44000, x * 32000, x * 30000, Western blot analysis Capsicum chinense

Synonyms

Synonyms Comment Organism
phosphatidylinositol-specific phospholipase C
-
 Catharanthus roseus
phosphatidylinositol-specific phospholipase C
-
 Coffea arabica
phosphatidylinositol-specific phospholipase C
-
 Capsicum chinense
PI-PLC
-
 Catharanthus roseus
PI-PLC
-
 Coffea arabica
PI-PLC
-
 Capsicum chinense

pI Value

Organism Comment pI Value Maximum pI Value
Coffea arabica sequence analysis
-
 5.31