Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 1 µM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
Mg2+ | hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
neomycin | hydrolysis of phosphatidylinositol and 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii | |
substance 48/80 | inhibition is more pronounced with phosphatidylinositol as substrate than with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
phosphatidylinositol | recombinant enzyme | Toxoplasma gondii | |
0.13 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | recombinant enzyme | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | extracellular phase of plasma membrane | Toxoplasma gondii | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | when phosphatidylinositol is used as a substrate, the enzyme activity increases with increasing free Ca2+ concentrations above 0.001 mM, and the activity decreases when free Ca2+-concentration exceeded 1 mM. When 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is used as substrate, the enzyme is activated at lower Ca2+-concentrations and its activity increases with increasing free Ca2+ concentration. Addition of 3 mM MgCl2 stimulates hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and inhibites hydrolysis of phosphatidylinositol | Toxoplasma gondii | |
Mg2+ | slight stimulation of hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
121000 | - |
calculated from sequence | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q3ZTU7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Toxoplasma gondii | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
phosphatidylinositol + H2O | - |
Toxoplasma gondii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TgPI-PLC | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
30 | - |
hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 30 | 5°C: about 50% of maximal activity, 30°C: about 45% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |
15 | 37 | 15°C: about 75% of maximal activity, 37°C: about 80% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.5 | hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
8 | - |
hydrolysis of phosphatidylinositol | Toxoplasma gondii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.6 | pH 7.1: about 85% of maximal activity, pH 7.6: about 65% of maximal activity, hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Toxoplasma gondii |
7.1 | 9.2 | pH 7.1: about 90% of maximal activity, pH 9.2: about 85% of maximal activity, hydrolysis of phosphatidylinositol | Toxoplasma gondii |