Literature summary for 3.1.4.11 extracted from

Venkataraman, G.; Goswami, M.; Tuteja, N.; Reddy, M.K.; Sopory, S.K.
Isolation and characterization of a phospholipase C delta isoform from pea that is regulated by light in a tissue specific manner (2003), Mol. Genet. Genomics, 270, 378-386.

Search for more literature for 3.1.4.11

Cloned(Commentary)

Cloned (Comment)	Organism
PLC delta isoform, expression in Escherichia coli BRL (DE3), sequencing, genomic organization	Pisum sativum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inclusion body	recombinant PLC delta isoform expressed in Escherichia coli	Pisum sativum	16234	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	coordinated at the active site	Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
67440	-	x * 67440, PLC delta isoform, calculated from the amino acid sequence	Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Pisum sativum	PsPLC delta isoform is regulated by light in a tissue specific manner and may be involved in important functions	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	O24297	PsPLC delta isoform	-

Purification (Commentary)

Purification (Comment)	Organism
from inclusion bodies, inactive recombinant PLC delta isoform, may be due to an incorrect refolding	Pisum sativum

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the PsPLC delta isoform gene is expressed in all pea tissues, expression is regulated by light in a tissue specific manner	Pisum sativum	-
root	higher expression of PsPLC delta isoform in roots than in shoots	Pisum sativum	-
seedling	light-grown	Pisum sativum	-
shoot	lower expression of PsPLC delta isoform in shoots than in roots	Pisum sativum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O	-	Pisum sativum	1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol	-	?
additional information	PsPLC delta isoform is regulated by light in a tissue specific manner and may be involved in important functions	Pisum sativum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 67440, PLC delta isoform, calculated from the amino acid sequence	Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Pisum sativum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.2	-	assay at	Pisum sativum

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Release 2023.1 (January 2023)