Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | TcPI-PLC expression is induced during the differentiation of trypomastigotes into amastigotes | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3), sequencing | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | pH 7.1, 30°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | associated with, in amastigotes | Trypanosoma cruzi | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on free Ca2+, enzyme needs a higher calcium concentration for its activation with phosphatidylinositol as substrate than with phosphatidylinositol 4,5-bisphosphate | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis | Trypanosoma cruzi |
82000 | - |
x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | Trypanosoma cruzi | inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q9TZN8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | TcPI-PLC is lipid modified in vivo, TcPI-PLC contains an N-myristoylation consensus sequence at its N-terminal end and is modified by myristate and palmitate, myristoyl group may play an important role in the association of enzyme with the plasma membrane | Trypanosoma cruzi |
Purification (Comment) | Organism |
---|---|
recombinant TcPI-PLC | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | TcPI-PLC expression at high levels in the epimastigote and amastigote stages | Trypanosoma cruzi | - |
epimastigote | TcPI-PLC expression at high levels in the epimastigote and amastigote stages | Trypanosoma cruzi | - |
trypomastigote | lower TcPI-PLC expression than in epimastigotes and amastigotes | Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | phosphoinositide-specific, preferred substrate to phosphatidylinositol, domain structure | Trypanosoma cruzi | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | inositol phosphate/diacylglycerol signaling pathway, TcPI-PLC is lipid modified in vivo, TcPI-PLC is developmentally regulated, its expression is induced during the differentiation of trypomastigotes into amastigotes at an early stage in the transformation process | Trypanosoma cruzi | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
phosphatidylinositol + H2O | phosphatidylinositol 4,5-bisphosphate is preferred to phosphatidylinositol | Trypanosoma cruzi | myo-inositol 1-phosphate + diacylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, calculated from the amino acid sequence, x * 80000, SDS-PAGE, Western blot analysis | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
TcPI-PLC | PI-PLC of Trypanosoma cruzi, similar to mamalian delta-type PI-PLCs | Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 6.8 | - |
Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.3 | enzyme activity decreases very rapidly below pH 6.5 and above pH 7.25 | Trypanosoma cruzi |