Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Epidermal growth factor | stimulates PLCepsilon overexpressed in COS-7 cells | Rattus norvegicus | |
additional information | study of PLCepsilon activation by several Ras mutants, recombinant purified PLCepsilon is not activated by Galpha-q, Gbetagamma or H-Ras in vitro | Rattus norvegicus | |
Q61L H-Ras | constitutively active Q61L H-Ras stimulates PLCepsilon co-expressed in COS-7 cells in parallel with Ras binding | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
PLCepsilon, expression in Sf-9 cells and in COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K2150A | mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras | Rattus norvegicus |
K2150E | mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras | Rattus norvegicus |
K2152A | mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras | Rattus norvegicus |
K2152E | mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras | Rattus norvegicus |
additional information | PLCepsilon mutants with deletions of one of the two Ras-binding domains, no activation by Q61L H-Ras | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+-dependent, maximum activity at 800 nM free Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
255000 | - |
x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways | ? | - |
? | |
additional information | Rattus norvegicus PLCepsilon | PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q99P84 | PLCepsilon | - |
Rattus norvegicus PLCepsilon | Q99P84 | PLCepsilon | - |
Purification (Comment) | Organism |
---|---|
recombinant PLCepsilon | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | expression of PLCepsilon | Rattus norvegicus | - |
liver | expression of PLCepsilon | Rattus norvegicus | - |
lung | expression of PLCepsilon | Rattus norvegicus | - |
skeletal muscle | expression of PLCepsilon | Rattus norvegicus | - |
small intestine | expression of PLCepsilon | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Rattus norvegicus | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Rattus norvegicus PLCepsilon | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
additional information | PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains | Rattus norvegicus | ? | - |
? | |
additional information | PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways | Rattus norvegicus | ? | - |
? | |
additional information | PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains | Rattus norvegicus PLCepsilon | ? | - |
? | |
additional information | PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways | Rattus norvegicus PLCepsilon | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | four PLC classes: beta, gamma and delta, which are differentially regulated by heterotrimeric G-proteins, tyrosine kinases and calcium, and epsilon | Rattus norvegicus |