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Literature summary for 3.1.4.11 extracted from

  • Kelley, G.G.; Reks, S.E.; Ondrako, J.M.; Smrcka, A.V.
    Phospholipase C(epsilon): a novel Ras effector (2001), EMBO J., 20, 743-754.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
Epidermal growth factor stimulates PLCepsilon overexpressed in COS-7 cells Rattus norvegicus
additional information study of PLCepsilon activation by several Ras mutants, recombinant purified PLCepsilon is not activated by Galpha-q, Gbetagamma or H-Ras in vitro Rattus norvegicus
Q61L H-Ras constitutively active Q61L H-Ras stimulates PLCepsilon co-expressed in COS-7 cells in parallel with Ras binding Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
PLCepsilon, expression in Sf-9 cells and in COS-7 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K2150A mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras Rattus norvegicus
K2150E mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras Rattus norvegicus
K2152A mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras Rattus norvegicus
K2152E mutation in the Ras-binding domain 2 of PLCepsilon, reduced binding of Q61L H-Ras Rattus norvegicus
additional information PLCepsilon mutants with deletions of one of the two Ras-binding domains, no activation by Q61L H-Ras Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-dependent, maximum activity at 800 nM free Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
255000
-
 x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways ?
-
 ?
additional information Rattus norvegicus PLCepsilon PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q99P84 PLCepsilon
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Rattus norvegicus PLCepsilon Q99P84 PLCepsilon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PLCepsilon Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
heart expression of PLCepsilon Rattus norvegicus
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liver expression of PLCepsilon Rattus norvegicus
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lung expression of PLCepsilon Rattus norvegicus
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skeletal muscle expression of PLCepsilon Rattus norvegicus
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small intestine expression of PLCepsilon Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Rattus norvegicus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Rattus norvegicus PLCepsilon 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
additional information PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains Rattus norvegicus ?
-
 ?
additional information PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways Rattus norvegicus ?
-
 ?
additional information PLCepsilon contains a GTP exchange factor and two C-terminal Ras-binding domains Rattus norvegicus PLCepsilon ?
-
 ?
additional information PLCepsilon is a Ras effector, directly regulated by Ras and links two critical signaling pathways Rattus norvegicus PLCepsilon ?
-
 ?

Subunits

Subunits Comment Organism
? x * 255000, PLCepsilon, calculated from the amino acid sequence and Western blot analysis Rattus norvegicus

Synonyms

Synonyms Comment Organism
More four PLC classes: beta, gamma and delta, which are differentially regulated by heterotrimeric G-proteins, tyrosine kinases and calcium, and epsilon Rattus norvegicus