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Literature summary for 3.1.4.11 extracted from

  • Sharom, F.J.; Lehto, M.T.
    Glycosylphosphatidylinositol-anchored proteins: structure, function, and cleavage by phosphatidylinositol-specific phospholipase C (2002), Biochem. Cell Biol., 80, 535-549.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information cleavage of GPI-anchored proteins by PI-PLC is influenced by bilayer fluidity, higher fluidity activates Bacillus thuringiensis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Listeria monocytogenes
free form, in complex with myo-inositol and with glucosaminyl(alpha1-6)-D-myo-inositol Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study of 5’-nucleotidase cleavage Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus thuringiensis
-
-
-
Clostridium novyi
-
-
-
Listeria monocytogenes
-
-
-
Staphylococcus aureus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol mechanism Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycosylphosphatidylinositol + H2O catalytic mechanism Bacillus cereus ?
-
?
additional information PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5’-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane Staphylococcus aureus ?
-
?
additional information PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5’-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane Bacillus thuringiensis ?
-
?
additional information PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5’-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane Clostridium novyi ?
-
?
additional information PI-PLC has a high affinity for the glycosylphosphatidylinositol anchor of GPI-anchored proteins, e.g. 5’-nucleotidase and alkaline phosphatase, and catalyzes cleavage in vitro to release a soluble protein and diacylglycerol, which remains in the membrane, enzyme structure Bacillus cereus ?
-
?
phosphatidylinositol + H2O phosphatidylinositol-specific, catalytic mechanism Bacillus cereus diacylglycerol + myo-inositol 1-phosphate
-
?