Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Murai, J.; Huang, S.Y.; Das, B.B.; Dexheimer, T.S.; Takeda, S.; Pommier, Y.
    Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs DNA damage induced by topoisomerases I and II and base alkylation in vertebrate cells (2012), J. Biol. Chem., 287, 12848-12857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in and functional complementation of Tdp1-knock-out Gallus gallus DT40 cells. The chicken Tdp1-/- cells complemented with human enzyme show a similar amount of TDP1 protein expression compared with human 293T cells Homo sapiens
recombinant expression of Homo sapiens enzyme and functional complementation of Tdp1-knock-out Gallus gallus DT40 cells Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
DT-40 cell
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant human enzyme processes 5'-phosphotyrosyl DNA ends when they mimic the 5'-overhangs of topoisomerase II-DNA cleavage complexes and 3'-deoxyribose phosphates generated from hydrolysis of abasic sites Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TDP1
-
Gallus gallus
TDP1
-
Homo sapiens
tyrosyl-DNA phosphodiesterase 1
-
Gallus gallus
tyrosyl-DNA phosphodiesterase 1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Gallus gallus
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Gallus gallus
7.5
-
assay at Homo sapiens

Expression

Organism Comment Expression
Gallus gallus generation of single Tdp1-knock-out Gallus gallus DT40 cells and of dual Tdp1-CtIP-deficient DT40 cells additional information

General Information

General Information Comment Organism
malfunction Tdp1-knock-out Gallus gallus DT40 cells are hypersensitive to camptothecin and bleomycin but also to etoposide, methyl methanesulfonate, H2O2, and ionizing radiation and they are deficient in mitochondrial enzyme activity, phenotype, overview Gallus gallus
physiological function tyrosyl-DNA phosphodiesterase 1 repairs topoisomerase I cleavage complexes by hydrolyzing their 3'-phosphotyrosyl DNA bonds and repairs bleomycin-induced DNA damage by hydrolyzing 3'-phosphoglycolates Homo sapiens
physiological function tyrosyl-DNA phosphodiesterase 1 repairs topoisomerase I cleavage complexes by hydrolyzing their 3'-phosphotyrosyl DNA bonds and repairs bleomycin-induced DNA damage by hydrolyzing 3'-phosphoglycolates. The yeast enzyme is also implicated in the repair of topoisomerase II-DNA cleavage complexes. The enzyme and CtIP act in parallel pathways for the repair of topoisomerase I cleavage complexes and methyl methanesulfonate-induced lesions but are epistatic for topoisomerase II cleavage complexes Gallus gallus