Cloned (Comment) | Organism |
---|---|
recombinant expression in and functional complementation of Tdp1-knock-out Gallus gallus DT40 cells. The chicken Tdp1-/- cells complemented with human enzyme show a similar amount of TDP1 protein expression compared with human 293T cells | Homo sapiens |
recombinant expression of Homo sapiens enzyme and functional complementation of Tdp1-knock-out Gallus gallus DT40 cells | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
DT-40 cell | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant human enzyme processes 5'-phosphotyrosyl DNA ends when they mimic the 5'-overhangs of topoisomerase II-DNA cleavage complexes and 3'-deoxyribose phosphates generated from hydrolysis of abasic sites | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDP1 | - |
Gallus gallus |
TDP1 | - |
Homo sapiens |
tyrosyl-DNA phosphodiesterase 1 | - |
Gallus gallus |
tyrosyl-DNA phosphodiesterase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Gallus gallus |
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Gallus gallus | generation of single Tdp1-knock-out Gallus gallus DT40 cells and of dual Tdp1-CtIP-deficient DT40 cells | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | Tdp1-knock-out Gallus gallus DT40 cells are hypersensitive to camptothecin and bleomycin but also to etoposide, methyl methanesulfonate, H2O2, and ionizing radiation and they are deficient in mitochondrial enzyme activity, phenotype, overview | Gallus gallus |
physiological function | tyrosyl-DNA phosphodiesterase 1 repairs topoisomerase I cleavage complexes by hydrolyzing their 3'-phosphotyrosyl DNA bonds and repairs bleomycin-induced DNA damage by hydrolyzing 3'-phosphoglycolates | Homo sapiens |
physiological function | tyrosyl-DNA phosphodiesterase 1 repairs topoisomerase I cleavage complexes by hydrolyzing their 3'-phosphotyrosyl DNA bonds and repairs bleomycin-induced DNA damage by hydrolyzing 3'-phosphoglycolates. The yeast enzyme is also implicated in the repair of topoisomerase II-DNA cleavage complexes. The enzyme and CtIP act in parallel pathways for the repair of topoisomerase I cleavage complexes and methyl methanesulfonate-induced lesions but are epistatic for topoisomerase II cleavage complexes | Gallus gallus |