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Literature summary for 3.1.4.1 extracted from

  • Kaneko, K.; Yamada, C.; Yanagida, A.; Koshu, T.; Umezawa, Y.; Itoh, K.; Hori, H.; Mitsui, T.
    Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 3 (2011), Plant Biotechnol., 28, 69-76.
No PubMed abstract available
Search for more literature for 3.1.4.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Oryza sativa
DNA and amino acid sequence determination and analysis, expression of GFP-tagged full-length isozyme NPP1 and truncated mutants in Oryza sativa cells Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme NPP1 contains a plastid-targeting signal Oryza sativa 9507
-
additional information no targeting of isozyme NPP3 to plastids Oryza sativa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, mature isozyme NPP1, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryza sativa NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability ?
-
 ?
additional information Oryza sativa unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q10Q09 isozyme NPP3; cv. Nipponbare, isozyme NPP3
-
Oryza sativa Q6ZI95 isozyme NPP1; cv. Nipponbare, isozyme NPP1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification clear difference between N-terminal proteolytic processing schemes to form mature enzyme proteins of isozymes NPP1 and NPP3 Oryza sativa
proteolytic modification clear difference between N-terminal proteolytic processing schemes to form mature enzyme proteins of isozymes NPP1 and NPP3. NPP1 is matured to a 70-kDa protein by two-step proteolytic processing, proprotein processing occurs post-translationally in the ER-Golgi system, overview Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NPP1 exhibits hydrolytic activity toward ADP-glucose and plastid-targeting ability Oryza sativa ?
-
 ?
additional information unlike isozyme NPP1, isozyme NPP3 exhibits no hydrolytic activity toward ADP-glucose and no plastid-targeting ability Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, mature isozyme NPP1, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
NPP1
-
 Oryza sativa
NPP3
-
 Oryza sativa
nucleotide pyrophosphatase/phosphodiesterase 1
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Oryza sativa