Protein Variants | Comment | Organism |
---|---|---|
D236A | strong decrease in KM value | Acetivibrio thermocellus |
D236N | strong decrease in KM value | Acetivibrio thermocellus |
D392E | transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase | Acetivibrio thermocellus |
D392N | transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase | Acetivibrio thermocellus |
H189A | transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase | Acetivibrio thermocellus |
H189D | transformation of metal and substrate specificity, mutant is a Mn2+-dependent phosphodiesterase. Strong stimulation of activity | Acetivibrio thermocellus |
H189E | transformation of substrate specificity, mutant is a Mn2+/Ni2+-dependent phosphodiesterase | Acetivibrio thermocellus |
H189Q | decrease in phosphodiesterase activity | Acetivibrio thermocellus |
H264A | strong stimulation of phosphodiesterase activity | Acetivibrio thermocellus |
H264N | strong stimulation of phosphodiesterase activity | Acetivibrio thermocellus |
H264Q | strong stimulation of phosphodiesterase activity | Acetivibrio thermocellus |
H376D | transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase | Acetivibrio thermocellus |
H376N | transformation of metal specificity, mutant is a Mn2+-dependent phosphodiesterase/monoesterase | Acetivibrio thermocellus |
R237A | strong stimulation of phosphodiesterase activity | Acetivibrio thermocellus |
R237E | strong stimulation of phosphodiesterase activity | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+-dependent phophomonoesterase activity requires two additional amino acid residues compared with Ni2+-dependent phosphomonoester activity. Mn2+-dependent phosphodiesterase activity requires one amino acid residues less than Ni2+-dependent phosphodiesterase activity | Acetivibrio thermocellus | |
Ni2+ | both Ni2+-dependent phophomonoesterase activity and Ni2+-dependent phosphodiesterase activity require the same seven amino acid residues of the enzyme | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
bifunctional phosphodiesterase/phosphomonoesterase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
bis-p-nitrophenyl phosphate + H2O | - |
Acetivibrio thermocellus | p-nitrophenol + p-nitrophenyl phosphate | processive two-step mechanism | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H264Q, presence of Ni2+ | Acetivibrio thermocellus | |
1.7 | - |
2',3'-cAMP | pH 8.0, 45°C, wild-type, presence of Ni2+ | Acetivibrio thermocellus | |
2.1 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H376D, presence of Mn2+ | Acetivibrio thermocellus | |
2.5 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D236A, presence of Ni2+ | Acetivibrio thermocellus | |
3 | 6 | 2',3'-cAMP | pH 8.0, 45°C, mutant H189D, presence of Mn2+ | Acetivibrio thermocellus | |
3.3 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H189Q, presence of Mn2+ | Acetivibrio thermocellus | |
3.6 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant R237K, presence of Ni2+ | Acetivibrio thermocellus | |
3.7 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H189A, presence of Mn2+ | Acetivibrio thermocellus | |
8.8 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D392E, presence of Mn2+ | Acetivibrio thermocellus | |
9.3 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D236N, presence of Ni2+ | Acetivibrio thermocellus | |
12 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H264N, presence of Ni2+ | Acetivibrio thermocellus | |
13.8 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D392N, presence of Mn2+ | Acetivibrio thermocellus | |
18 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H264A, presence of Ni2+ | Acetivibrio thermocellus | |
19.3 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant R237A, presence of Ni2+ | Acetivibrio thermocellus | |
23.2 | - |
2',3'-cAMP | pH 8.0, 45°C, wild-type, presence of Mn2+ | Acetivibrio thermocellus | |
33 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant R237Q, presence of Ni2+ | Acetivibrio thermocellus | |
40.5 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H376N, presence of Mn2+ | Acetivibrio thermocellus | |
43.5 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D236A, presence of Mn2+ | Acetivibrio thermocellus | |
52.3 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H264D, presence of Mn2+ | Acetivibrio thermocellus | |
65.9 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant D236N, presence of Mn2+ | Acetivibrio thermocellus | |
117.5 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H189E, presence of Mn2+ | Acetivibrio thermocellus | |
253 | - |
2',3'-cAMP | pH 8.0, 45°C, mutant H264Q, presence of Mn2+ | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
phosphomonoesterase activity, assay at | Acetivibrio thermocellus |
8 | - |
phosphodiesterase activity, assay at | Acetivibrio thermocellus |