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Literature summary for 3.1.4.1 extracted from
- Two low Km hydrolytic activities on dinucleoside 5',5'''-P1,P4-tetraphosphates in rat liver. Characterization as the specific dinucleoside tetraphosphatase and a phosphodiesterase-I-like enzyme (1984), J. Biol. Chem., 259, 2879-2885.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Rattus norvegicus |  |
| EDTA | inactivation reversed by Zn2+ | Rattus norvegicus | |
| glutathione | - |
Rattus norvegicus | |
| guanosine 5'-tetraphosphate | competitive | Rattus norvegicus | |
| L-cysteine | - |
Rattus norvegicus | |
| Mn2+ | inhibition at higher concentration, 0.1-0.5 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
diadenosine 5',5'''-P1,P4-tetraphosphate | - |
Rattus norvegicus | |
| 0.011 | - |
diadenosine 5',5'''-P1,P3-triphosphate | - |
Rattus norvegicus | |
| 0.012 | - |
diguanosine 5',5'''-P1,P4-tetraphosphate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Rattus norvegicus | |
| divalent cations | required for activity | Rattus norvegicus | |
| Mg2+ | - |
Rattus norvegicus | |
| Mn2+ | activation with low concentrations, 0.05 mM | Rattus norvegicus | |
| Zn2+ | required for activity | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O | - |
Rattus norvegicus | AMP + ATP | - |
? | |
| diadenosine 5',5'''-P1,P3-triphosphate + H2O | - |
Rattus norvegicus | AMP + ADP | - |
? | |
| diguanosine 5',5'''-P1,P4-tetraphosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| dinucleoside tetraphosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| additional information | no activity with cAMP | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with p-nitrophenylthymidine 3'-phosphate | Rattus norvegicus | ? | - |
? | |
| additional information | broad substrate specificity | Rattus norvegicus | ? | - |
? | |
| p-nitrophenylthymidine 5'-phosphate + H2O | - |
Rattus norvegicus | 5'-TMP + p-nitrophenol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
at pH 7.0 less than 10% activity of that at pH 8.5 | Rattus norvegicus |
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