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Literature summary for 3.1.4.1 extracted from
- Preparation of an exonuclease from Lactobacillus which releases 5'-mononucleotides from polynucleotides (1969), Biochemistry, 8, 4831-4837.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3'-CMP | stimulate hydrolysis | Lactobacillus acidophilus |  |
| dithiol-stabilizing agent | activation | Lactobacillus acidophilus |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes | Lactobacillus acidophilus |
| Mn2+ stabilizes, protects from irreversible inactivation: activity lost irreversibly when the enzyme preparation is dialyzed for 2 h against buffer that does not contain Mn2+ | Lactobacillus acidophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-deoxyribonucleotides | - |
Lactobacillus acidophilus | |
| 5'-nucleotides | - |
Lactobacillus acidophilus | |
| 5'-ribonucleotides | - |
Lactobacillus acidophilus | |
| DNA | - |
Lactobacillus acidophilus | |
| EDTA | - |
Lactobacillus acidophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
CpU | - |
Lactobacillus acidophilus | |
| 0.22 | - |
U(pU)6 | - |
Lactobacillus acidophilus | |
| 0.25 | - |
ApG | - |
Lactobacillus acidophilus | |
| 0.43 | - |
ApA | - |
Lactobacillus acidophilus | |
| 2.8 | - |
ApC | - |
Lactobacillus acidophilus | |
| 6 | - |
p-nitrophenylthymidine 5'-phosphate | - |
Lactobacillus acidophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Lactobacillus acidophilus | |
| Co2+ | no activation | Lactobacillus acidophilus | |
| divalent cations | required for activity | Lactobacillus acidophilus | |
| Mg2+ | slight activation | Lactobacillus acidophilus | |
| Mn2+ | activation | Lactobacillus acidophilus | |
| Zn2+ | no activation | Lactobacillus acidophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus acidophilus | - |
R26 | - |
| Lactobacillus acidophilus R26 | - |
R26 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Lactobacillus acidophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.4 | - |
- |
Lactobacillus acidophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ApA + H2O | - |
Lactobacillus acidophilus | 5'-AMP + adenosine | - |
? | |
| ApC + H2O | - |
Lactobacillus acidophilus | 5'-CMP + adenosine | - |
? | |
| ApG + H2O | - |
Lactobacillus acidophilus | 5'-GMP + adenosine | - |
? | |
| CpU + H2O | - |
Lactobacillus acidophilus | 5'-UMP + cytidine | - |
? | |
| dinucleoside monophosphates + H2O | - |
Lactobacillus acidophilus | mononucleoside 5'-phosphate | - |
? | |
| DNA + H2O | native and denatured DNA | Lactobacillus acidophilus | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
| additional information | no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP | Lactobacillus acidophilus | ? | - |
? | |
| additional information | no activity with p-nitrophenylthymidine 3'-phosphate | Lactobacillus acidophilus | ? | - |
? | |
| additional information | no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP | Lactobacillus acidophilus R26 | ? | - |
? | |
| additional information | no activity with p-nitrophenylthymidine 3'-phosphate | Lactobacillus acidophilus R26 | ? | - |
? | |
| oligonucleotides + H2O | - |
Lactobacillus acidophilus | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
| oligonucleotides + H2O | - |
Lactobacillus acidophilus R26 | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
| p-nitrophenylthymidine 5'-phosphate + H2O | - |
Lactobacillus acidophilus | 5'-TMP + p-nitrophenol | - |
? | |
| p-nitrophenylthymidine 5'-phosphate + H2O | - |
Lactobacillus acidophilus R26 | 5'-TMP + p-nitrophenol | - |
? | |
| polynucleotides + H2O | - |
Lactobacillus acidophilus | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
| polynucleotides + H2O | - |
Lactobacillus acidophilus R26 | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
| U(pU)6 + H2O | - |
Lactobacillus acidophilus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Lactobacillus acidophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | activities at 25°C: 58%, at 55°C: 16%, respectively, of that at 37°C | Lactobacillus acidophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Lactobacillus acidophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Lactobacillus acidophilus |
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