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Literature summary for 3.1.4.1 extracted from
- Partial purification and properties of phosphodiesterase I from hog kidney (1961), J. Biol. Chem., 236, 3031-3036.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-deoxyribonucleotides | end-product inhibition | Sus scrofa | |
| 5'-nucleotides | end-product inhibition | Sus scrofa |  |
| ADP | competitive | Sus scrofa | |
| AMP | product inhibition | Sus scrofa | |
| ATP | competitive | Sus scrofa | |
| Cu2+ | purified enzyme | Sus scrofa | |
| EDTA | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
p-nitrophenylthymidine 5'-phosphate | pH 9.1 | Sus scrofa | |
| 0.17 | - |
p-nitrophenylthymidine 5'-phosphate | pH 8.0 | Sus scrofa | |
| 0.26 | - |
3'-O-acetyl-p-nitrophenylthymidine 5'-phosphate | pH 8.0 | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Sus scrofa | 5764 | - |
| microsome | - |
Sus scrofa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight activation | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, kidney | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.5 | 29 | - |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-O-acetyl-5'-O-[hydroxy(4-nitrophenoxy)phosphoryl]thymidine + H2O | - |
Sus scrofa | p-nitrophenol + 3'-O-acetylthymidine 5'-phosphate | - |
? | |
| 5'-p-nitrophenyluridine 5'-phosphate + H2O | 70% as fast as 5'-p-nitrophenylthymidine 5'-phosphate | Sus scrofa | 5'-UMP + p-nitrophenol | - |
? | |
| ADP + H2O | slowly | Sus scrofa | AMP + phosphate | - |
? | |
| ATP + H2O | slowly | Sus scrofa | 5'-AMP + diphosphate | - |
? | |
| cyclic 3',5'-mononucleotides + H2O | slowly | Sus scrofa | mononucleoside 5'-phosphate | - |
? | |
| additional information | no activity with dinucleotides bearing 3'-phosphate | Sus scrofa | ? | - |
? | |
| additional information | no activity with p-nitrophenylthymidine 3'-phosphate | Sus scrofa | ? | - |
? | |
| oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Sus scrofa | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
| p-nitrophenylthymidine 5'-phosphate + H2O | - |
Sus scrofa | 5'-TMP + p-nitrophenol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 65 | - |
Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | - |
Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 66 | - |
stable below | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.1 | - |
Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.5 | - |
Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
inactivation below | Sus scrofa |
| 6 | 10 | stable between | Sus scrofa |
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