Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Fujimoto, M.; Fujiyama, K.; Midorikawa, Y.; Fujishima, T.; Kuninaka, A.; Yoshino, H.
    Properties of 5'-phosphodiesterase from Aspergillus niger (1977), Agric. Biol. Chem., 41, 737-744.
No PubMed abstract available
Search for more literature for 3.1.4.1

Application

Application Comment Organism
synthesis production of 5'-mononucleotides, degradation of 2',5'-dinucleotides in nuclease P1 digest of technical grade yeast RNA Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
terminal 3'-monophosphoryl group of substrates
-
 Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus batatae
-
-
-
Aspergillus carbonarius
-
-
-
Aspergillus fuscus
-
-
-
Aspergillus inuii
-
-
-
Aspergillus niger
-
-
-
Aspergillus phoenicis
-
-
-
Aspergillus usamii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, Aspergillus niger AHU 7117 Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus niger
additional information
-
-
 Aspergillus phoenicis
additional information
-
-
 Aspergillus awamori
additional information
-
-
 Aspergillus usamii
additional information
-
-
 Aspergillus carbonarius
additional information
-
-
 Aspergillus inuii
additional information
-
-
 Aspergillus fuscus
additional information
-
-
 Aspergillus batatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
-
 Aspergillus niger nucleoside 5'-monophosphate + p-nitrophenol
-
 ?
ADP + H2O
-
 Aspergillus niger AMP + phosphate
-
 ?
CDP-choline + H2O
-
 Aspergillus niger ?
-
 ?
dinucleoside monophosphates + H2O 3'-5' linkage Aspergillus niger mononucleoside 5'-phosphate
-
 ?
dinucleoside monophosphates + H2O no specificity toward bases or sugars Aspergillus niger mononucleoside 5'-phosphate
-
 ?
dinucleoside monophosphates + H2O 2'-5' linkage Aspergillus niger mononucleoside 5'-phosphate
-
 ?
dinucleotides + H2O 3'-hydroxyl-terminated 3'-5'and 2'-5' linkage without specificity toward bases or sugars Aspergillus niger ?
-
 ?
additional information no activity with dinucleotides bearing 3'-phosphate , 2': 3'-cyclic AMP and 3': 5'-cyclic AMP Aspergillus niger ?
-
 ?
oligonucleotides + H2O
-
 Aspergillus niger oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
p-nitrophenylthymidine 5'-phosphate + H2O
-
 Aspergillus niger 5'-TMP + p-nitrophenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable below Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.3 5.3 stable between Aspergillus niger