Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli | Staphylococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
V66K | the substitution affects the H/D exchange properties of the protein globally, even when Lys66 is neutral. The conformational dynamics of the protein probed by H/D exchange indicate that, while both the global fluctuation and the local fluctuation are increased by the substitution, the global fluctuations are enhanced by protonation of Lys-66 | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Staphylococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
SNase | - |
Staphylococcus sp. |
staphylococcal nuclease | - |
Staphylococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | presence and ionization of Lys66, buried in the hydrophobic core of a stabilized variant of staphylococcal nuclease, affect conformation and dynamics. The neutral Lys66 affects slow conformational fluctuations globally, whereas the effects of the charged form are localized to the region immediately surrounding position 66, when Lys66 is charged the protein expands, structural reorganization triggered by ionization of the internal Lys66, detailed overview | Staphylococcus sp. |