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Literature summary for 3.1.31.1 extracted from

  • Shan, L.; Tong, Y.; Xie, T.; Wang, M.; Wang, J.
    Restricted backbone conformational and motional flexibilities of loops containing peptidyl-proline bonds dominate the enzyme activity of staphylococcal nuclease (2007), Biochemistry, 46, 11504-11513.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
proline free mutant Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
P11A/P31A/P42A/P47T/P56A/P117G proline free mutant, conformationally different from wild type protein, 1.4% of wild type activity Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P00644
-
-

Purification (Commentary)

Purification (Comment) Organism
proline free mutant Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ss-DNA + H2O single strand salmon sperm DNA, obtained by boiling for 30 min and rapid cooling on ice Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
staphylococcal nuclease
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Staphylococcus aureus