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Literature summary for 3.1.31.1 extracted from

  • Chen, H.M.; Chan, S.C.; Leung, K.W.; Wu, J.M.; Fang, H.J.; Tsong, T.Y.
    Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding (2005), FEBS J., 272, 3967-3974.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D143G Tm value for mutant enzyme is 50.53°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D146G Tm value for mutant enzyme is 50.99°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D19G Tm value for mutant enzyme is 52.06°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D21G Tm value for mutant enzyme is 53.74°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D40G Tm value for mutant enzyme is 50.44°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D77G Tm value for mutant enzyme is 44.14°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D83G Tm value for mutant enzyme is 37.21°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
D95G Tm value for mutant enzyme is 37.38°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E101G Tm value for mutant enzyme is 43.04°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E10G Tm value for mutant enzyme is 43.8°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E122G Tm value for mutant enzyme is 44.12°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E129G Tm value for mutant enzyme is 34.59°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E135G Tm value for mutant enzyme is 44.54°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E142G Tm value for mutant enzyme is 49.41°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E43G Tm value for mutant enzyme is 54.99°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E52G Tm value for mutant enzyme is 52.1°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E57G Tm value for mutant enzyme is 46.6°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E67G Tm value for mutant enzyme is 46.53°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E73G Tm value for mutant enzyme is 37°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.
E75G Tm value for mutant enzyme is 36.99°C, compared to 50.98°C for wild-type enzyme Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
recombinant
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
Tm-value for wild-type enzyme is 30.98°C Staphylococcus sp.