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Literature summary for 3.1.31.1 extracted from

  • Li, Y.; Jing, G.
    Double point mutant F34W/W140F of staphylococcal nuclease is in a molten globule state but highly competent to fold into a functional conformation (2000), J. Biochem., 128, 739-744.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F34W/W140F characterized by far and near UV CD, gel filtration, ANS-binding fluorescence, enzymatic parameters are similar to those of the wild type, similar substrate affinity to the wild type enzyme Staphylococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00409
-
DNA F34W/W140F mutant, 25°C, pH 7.4 Staphylococcus sp.
0.00431
-
DNA wild type, 25°C, pH 7.4 Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA
-
Staphylococcus sp. ?
-
?