Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.31.1 extracted from

  • Anfinsen, C.B; Cuatrecasas, P.; Taniuchi, H.
    Staphylococcal nuclease chemical properties and catalysis (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 177-204.
No PubMed abstract available
Search for more literature for 3.1.31.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ competitive Staphylococcus aureus
Cu2+ competitive Staphylococcus aureus
Hg2+ competitive Staphylococcus aureus
Mononucleotides with 5'-phosphate end group Staphylococcus aureus
oligonucleotides with 5'-phosphate end group Staphylococcus aureus
Zn2+ competitive Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Staphylococcus aureus
Cu2+ minimal activation if Ca2+ is replaced by Cu2+ Staphylococcus aureus
Fe2+ minimal activation if Ca2+ is replaced by Fe2+ Staphylococcus aureus
Sr2+ DNase but no RNase activity if Ca2+ is replaced Sr2+ Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus Foggi Worthington
-
-
-
Staphylococcus aureus V8
-
-
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products mechanism Staphylococcus aureus
a

Renatured (Commentary)

Renatured (Comment) Organism
high concentration of urea and guanidinium chloride denaturation is completely reversible at pH 3.5-3.7, 55-65°C Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-O-acetylnitrophenyl-pdT + H2O
-
 Staphylococcus aureus ?
-
 ?
5'-chloromethyl-pdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
5'-O-acetyl-dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
5'-sulfate-dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
 ?
DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
 ?
DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides
-
 ?
DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus V8 3'-deoxymononucleotides + dinucleotides
-
 ?
DNA + H2O in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides
-
 ?
DNA + H2O denaturated DNA is hydrolyzed more rapidly than native DNA Staphylococcus aureus Foggi Worthington 3'-deoxymononucleotides + dinucleotides
-
 ?
dTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
methyl-pdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
additional information specificity Staphylococcus aureus ?
-
 ?
additional information specificity Staphylococcus aureus V8 ?
-
 ?
additional information specificity Staphylococcus aureus Foggi Worthington ?
-
 ?
nitrophenyl-pdT + H2O
-
 Staphylococcus aureus ?
-
 ?
nitrophenyl-pdT + H2O
-
 Staphylococcus aureus V8 ?
-
 ?
nitrophenyl-pdT + H2O
-
 Staphylococcus aureus Foggi Worthington ?
-
 ?
nitrophenyl-pdTp + H2O
-
 Staphylococcus aureus ?
-
 ?
nitrophenyl-pdTpdTp-nitrophenyl + H2O
-
 Staphylococcus aureus ?
-
 ?
RNA + H2O
-
 Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides
-
 ?
RNA + H2O
-
 Staphylococcus aureus V8 nucleoside 3'-phosphates + dinucleotides
-
 ?
RNA + H2O
-
 Staphylococcus aureus Foggi Worthington nucleoside 3'-phosphates + dinucleotides
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH optimum depends on Ca2+ concentration Staphylococcus aureus

pH Range

pH Minimum pH Maximum Comment Organism
9 10 depends on Ca2+ concentration Staphylococcus aureus