Literature summary for 3.1.30.2 extracted from

Caballero, I.; Piedrahita, J.A.
Evaluation of the Serratia marcescens nuclease (NucA) as a transgenic cell ablation system in porcine (2009), Anim. Biotechnol., 20, 177-185.

Search for more literature for 3.1.30.2

Cloned(Commentary)

Cloned (Comment)	Organism
construct containing the NucA gene driven by the beta-actin promoter coupled with enhancer elements from the cytomegalovirus promoter and rabbit beta-globin gene and the blasticidin resistance gene driven by the phosphoglycerate kinase promoter are generated and electroporated into porcine fetal fibroblasts	Serratia marcescens

Organism

Organism	UniProt	Comment	Textmining
Serratia marcescens	P13717	-	-

Synonyms

Synonyms	Comment	Organism
nucA	-	Serratia marcescens
NucANLS	-	Serratia marcescens
NucANLS	gene with a nuclear localization signal	Serratia marcescens
Serratia marcescens nuclease	-	Serratia marcescens

Expression

Organism	Comment	Expression
Serratia marcescens	both NucA with and without a nuclear localization signal prove to be effective in killing porcine fibroblasts compared to controls, gene expression analysis of surviving colonies indicates that survival is related to low or absent expression of the toxic genes	additional information

General Information

General Information	Comment	Organism
physiological function	enzyme is able to ablate cells in culture	Serratia marcescens

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Release 2023.1 (January 2023)