Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia phage T7 |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia phage T7 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation in beta-bridge center that separates the catalytic domains, 20-50 times lower nonspecific nuclease activity in Mg2+ buffer | Escherichia phage T7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for resolving of cruciform structures | Escherichia phage T7 | |
Mn2+ | 20times lower activity in resolving of cruciform structures than with Mg2+ | Escherichia phage T7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia phage T7 | T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures | nicked DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant T7 endonuclease | Escherichia phage T7 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.6-0.8 U/mg, 1 unit is defined as the amount of enzmyme that is needed to convert 0.001 mg of supercoiled pUC(AT) to either the linear or nicked form in a 0.02 ml reaction at 37°C in 30 min | Escherichia phage T7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures | Escherichia phage T7 | nicked DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia phage T7 |
Synonyms | Comment | Organism |
---|---|---|
resolvase T7 | - |
Escherichia phage T7 |
T7 endo I | - |
Escherichia phage T7 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia phage T7 | deduced from nucleotide sequence | - |
9.5 |