Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxychinoline 5-sulfonate | with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA | Pisum sativum | |
Hg2+ | complete inactivation by 5 mM HgCl2 | Pisum sativum | |
NaCl | inhibition at concentrations higher than 100 mM | Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 60% stimulation of activity on denaturated DNA by 5 mM CaCl2 | Pisum sativum | |
Mg2+ | 40% stimulation of activity on denaturated DNA by 5 mM MgCl2 | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Pisum sativum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
dsDNA + H2O | 15-20fold higher activity on single-stranded than native DNA | Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
ssDNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
with denaturated DNA as substrate | Pisum sativum |
60 | - |
with native DNA or 3'-AMP as substrate | Pisum sativum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in Tris-HCl-buffer, at this pH only 30% of this activity in potassium phosphate buffer, only 60% in sodium citrate buffer | Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Pisum sativum |