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Literature summary for 3.1.30.1 extracted from

  • Wani, A.A.; Hadi, S.M.
    Partial purification and properties of an endonuclease from germinating pea seeds specific for single-stranded DNA (1979), Arch. Biochem. Biophys., 196, 138-146.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxychinoline 5-sulfonate with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA Pisum sativum
Hg2+ complete inactivation by 5 mM HgCl2 Pisum sativum
NaCl inhibition at concentrations higher than 100 mM Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 60% stimulation of activity on denaturated DNA by 5 mM CaCl2 Pisum sativum
Mg2+ 40% stimulation of activity on denaturated DNA by 5 mM MgCl2 Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Pisum sativum
-
5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
dsDNA + H2O 15-20fold higher activity on single-stranded than native DNA Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?
ssDNA + H2O
-
Pisum sativum 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
with denaturated DNA as substrate Pisum sativum
60
-
with native DNA or 3'-AMP as substrate Pisum sativum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
in Tris-HCl-buffer, at this pH only 30% of this activity in potassium phosphate buffer, only 60% in sodium citrate buffer Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
Pisum sativum