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Literature summary for 3.1.30.1 extracted from

  • Otto, B.; Knippers, R.
    An endonuclease from mouse cells specific for single-stranded DNA (1976), Eur. J. Biochem., 71, 617-622.
    View publication on PubMed
Search for more literature for 3.1.30.1

Activating Compound

Activating Compound Comment Organism Structure
dimethyl sulfoxide
-
 Mus musculus
glycerol 2fold stimulation of activity by 10-20% in 50 mM Tris-HCl-buffer, pH 7.5 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 30% of activity towards denaturated ssDNA with 1.2 mM Mus musculus
Mg2+ concentrations higher 2 mM Mus musculus
NaCl complete inhibition with 50 mM Mus musculus
sodium dodecylsulfate complete inhibition with 0.2% Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration 1-2 mM MgCl2 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Mus musculus
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
ascites tumor cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 with 10 mM sodium phosphate buffer Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
 Mus musculus