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Literature summary for 3.1.30.1 extracted from

  • Martin, S.A.; Ullrich, R.C.; Meyer, W.L.
    Partial purification and properties of a nuclease from Schizophyllum commune with a preference toward single-stranded nucleic acid (1986), Biochim. Biophys. Acta, 867, 67-75.
No PubMed abstract available
Search for more literature for 3.1.30.1

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxychinoline 5-sulfonate 50% of activity towards ssDNA with 0.005 mM Schizophyllum commune
citrate at 0.2 M 50% of initial activity with ssDNA Schizophyllum commune
EDTA 50% of activity towards ssDNA with 0.0003 mM Schizophyllum commune
F- at 0.2 M 30% of initial activity with ssDNA Schizophyllum commune
heparin
-
 Schizophyllum commune
Ionic strength inhibition of activity at ionic strength above 0.3 M Schizophyllum commune
phosphate
-
 Schizophyllum commune
Polyvinyl sulfate
-
 Schizophyllum commune
sodium bisphosphate at 0.2 M 10% of initial activity with ssDNA Schizophyllum commune
Zn2+ 54% of initial activity towards ssDNA with 0.1 mM Zn2+; almost complete inactivation at 1.0 mM Schizophyllum commune

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at 0.0014 mM 105% restoration of activity after EDTA-inactivation Schizophyllum commune
Mn2+ at 0.0014 mM 53% restoration of activity after EDTA-inactivation Schizophyllum commune
Zn2+ stimulation of activity towards ssDNA at 0.001-0.01 mM, at 0.0014 mM 103% restoration of activity after EDTA-inactivation Schizophyllum commune

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
-
 Schizophyllum commune

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Schizophyllum commune
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
RNA + H2O Schizophyllum commune
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Schizophyllum commune
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schizophyllum commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
dsDNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
RNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
ssDNA + H2O
-
 Schizophyllum commune 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

Synonyms Comment Organism
SC nuclease
-
 Schizophyllum commune

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Schizophyllum commune

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 50% of activity with ssDNA at 47°C, with dsDNA at 49°C Schizophyllum commune

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 50% of optimal activity towards ssDNA and dsDNA Schizophyllum commune

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 in 30 mM buffer with 200 mM NaCl, more stable above pH 7.0 than below Schizophyllum commune