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Literature summary for 3.1.3.99 extracted from
- Purification and some properties of an IMP-specific 5'-nucleotidase from yeast (1994), Biochem. J., 298, 593-598.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 121% activity at 2.5 mM | Saccharomyces cerevisiae |  |
| ATP | 243% activity at 2.5 mM | Saccharomyces cerevisiae | |
| cyclic AMP | 130% activity at 2.5 mM | Saccharomyces cerevisiae | |
| diadenosine pentaphosphate | 252% activity at 2.5 mM | Saccharomyces cerevisiae | |
| Diadenosine tetraphosphate | 283% activity at 2.5 mM | Saccharomyces cerevisiae | |
| diadenosine triphosphate | 200% activity at 2.5 mM | Saccharomyces cerevisiae | |
| additional information | not stimulated by AMP, GTP, CTP, adenosine 5'-[alpha,beta-methylene]diphosphate, and 2,3-bisphosphoglycerate | Saccharomyces cerevisiae | |
| UTP | 139% activity at 2.5 mM | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | 86% residual activity at 2.5 mM | Saccharomyces cerevisiae | |
| GTP | 75% residual activity at 2.5 mM | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | may substitute Mg2+ | Saccharomyces cerevisiae | |
| Mg2+ | enzyme activity is absolutely dependent on bivalent metals with Mg2+ as most potent one | Saccharomyces cerevisiae | |
| Mn2+ | may substitute Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55400 | - |
2 * 55400, SDS-PAGE | Saccharomyces cerevisiae |
| 220000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IMP + H2O | Saccharomyces cerevisiae | highly specific substrate | inosine + phosphate | - |
? | |
| additional information | Saccharomyces cerevisiae | no activity with AMP, dIMP, GMP, XMP, UMP, dTMP, CMP, 2'(3')-IMP, D-ribose 5-phosphate, phenyl phosphate, and beta-glyerophosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE-Toyopearl column chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.056 | - |
crude extract, at pH 6.5 and 37°C | Saccharomyces cerevisiae |
| 102.5 | - |
after 1830.4fold purification, at pH 6.5 and 37°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IMP + H2O | highly specific substrate | Saccharomyces cerevisiae | inosine + phosphate | - |
? | |
| additional information | no activity with AMP, dIMP, GMP, XMP, UMP, dTMP, CMP, 2'(3')-IMP, D-ribose 5-phosphate, phenyl phosphate, and beta-glyerophosphate | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 2 * 55400, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMP-specific 5-nucleotidase | - |
Saccharomyces cerevisiae |
| Isn1 | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | with 100 mM imidazole/HCl buffer or 100 mM sodium acetate buffer in the presence of 25 mM Mg2+ | Saccharomyces cerevisiae |
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