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Literature summary for 3.1.3.93 extracted from

  • Laing, W.A.; Bulley, S.; Wright, M.; Cooney, J.; Jensen, D.; Barraclough, D.; MacRae, E.
    A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis (2004), Proc. Natl. Acad. Sci. USA, 101, 16976-16981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.93

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Actinidia deliciosa

Inhibitors

Inhibitors Comment Organism Structure
EDTA no activity is found in the presence of 0.2 mM EDTA without added Mg2+, native enzyme Actinidia deliciosa
LiCl inhibition of recombinant enzyme Actinidia deliciosa
Mg2+ above 2 mM, native enzyme Actinidia deliciosa
Mg2+
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 1.8 mM Mg2+, native enzyme Actinidia deliciosa
0.022
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 4.8 mM Mg2+ Actinidia deliciosa
0.044
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 2 mM Mg2+ Arabidopsis thaliana
0.15
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, recombinant enzyme Actinidia deliciosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely dependent on Mg2+. K1 value: 0.016 mM Arabidopsis thaliana
Mg2+ completely dependent on Mg2+ for activity, Ka-value: 0.2 mM Actinidia deliciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29121
-
 x * 29121, calculated from sequence Arabidopsis thaliana
29226
-
 2 * 29226, calculated from sequence Actinidia deliciosa
65000
-
 gel filtration, native enzyme Actinidia deliciosa

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa Q5U789
-
-
Arabidopsis thaliana Q9M8S8
-
-
Arabidopsis thaliana Columbia-1 Q9M8S8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Actinidia deliciosa
-
shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Arabidopsis thaliana L-galactose + phosphate
-
 ?
beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Actinidia deliciosa L-galactose + phosphate
-
 ?
beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 29121, calculated from sequence Arabidopsis thaliana
dimer 2 * 29226, calculated from sequence Actinidia deliciosa

Synonyms

Synonyms Comment Organism
At3g02870
-
 Arabidopsis thaliana
IMPL2 gene name, ambiguous Actinidia deliciosa
L-Gal-1-P phosphatase
-
 Arabidopsis thaliana
L-Gal-1-P phosphatase
-
 Actinidia deliciosa
L-Gal-1-Pase
-
 Actinidia deliciosa
VTC4 gene name, ambiguous Arabidopsis thaliana
VTC4 gene name, ambiguous Actinidia deliciosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7 at 2 mM MgCl2 and 0.5 mM L-galactose 1-phosphate Arabidopsis thaliana
7
-
-
 Actinidia deliciosa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.8 pH 6.5: about 70% of maximal activity, pH 7.8: about 0% of maximal activity Actinidia deliciosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 Mg2+ pH 7.0, 30°C, inhibition of native enzyme Actinidia deliciosa
0.62
-
 Mg2+ pH 7.0, 30°C Arabidopsis thaliana
3.7
-
 LiCl pH 7.0, 30°C, inhibition of recombinant enzyme Actinidia deliciosa

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated from sequence
-
 5.01