BRENDA - Enzyme Database
show all sequences of 3.1.3.89

General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli. Biochemical Characterization of SurE, YfbR, and Yjjg

Proudfoot, M.; Kuznetsova, E.; Brown, G.; Rao, N.N.; Kitagawa, M.; Mori, H.; Savchenko, A.; Yakunin, A.F.; J. Biol. Chem. 279, 54687-54694 (2004)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
0.2 mM, 21% residual activity
Escherichia coli
ATP
0.2 mM, 18% residual activity
Escherichia coli
CTP
0.2 mM, 29% residual activity
Escherichia coli
dATP
0.2 mM, 15% residual activity
Escherichia coli
dCTP
0.2 mM, 19% residual activity
Escherichia coli
dGTP
0.2 mM, 13% residual activity
Escherichia coli
dTTP
0.2 mM, 15% residual activity
Escherichia coli
GTP
0.2 mM, 28% residual activity
Escherichia coli
ITP
0.2 mM, 29% residual activity
Escherichia coli
TTP
0.2 mM, 14% residual activity
Escherichia coli
UTP
0.2 mM, 26% residual activity
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.012
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.017
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.02
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.036
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.047
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
2.09
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM
Escherichia coli
Cu2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
Mn2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
and tetramer, 3 * 24000, SDS-PAGE; and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
71300
-
and above 100000, gel filtration
Escherichia coli
100000
-
and 71300, gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
5'-dCMP + H2O
-
656266
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
?
5'-dTMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
additional information
enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates
656266
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
trimer
and tetramer, 3 * 24000, SDS-PAGE
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.2
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.23
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.23
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.24
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.3
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.56
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
0.2 mM, 21% residual activity
Escherichia coli
ATP
0.2 mM, 18% residual activity
Escherichia coli
CTP
0.2 mM, 29% residual activity
Escherichia coli
dATP
0.2 mM, 15% residual activity
Escherichia coli
dCTP
0.2 mM, 19% residual activity
Escherichia coli
dGTP
0.2 mM, 13% residual activity
Escherichia coli
dTTP
0.2 mM, 15% residual activity
Escherichia coli
GTP
0.2 mM, 28% residual activity
Escherichia coli
ITP
0.2 mM, 29% residual activity
Escherichia coli
TTP
0.2 mM, 14% residual activity
Escherichia coli
UTP
0.2 mM, 26% residual activity
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.012
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.017
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.02
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.036
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.047
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
2.09
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM
Escherichia coli
Cu2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
Mn2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
and tetramer, 3 * 24000, SDS-PAGE; and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
71300
-
and above 100000, gel filtration
Escherichia coli
100000
-
and 71300, gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
5'-dCMP + H2O
-
656266
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
?
5'-dTMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
additional information
enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates
656266
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
trimer
and tetramer, 3 * 24000, SDS-PAGE
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.2
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.23
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.23
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.24
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.3
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
0.56
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
-
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.27
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
4.22
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
6.42
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
11.6
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
14.1
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
21.3
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
25.6
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.27
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
4.22
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
6.42
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
11.6
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
14.1
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
21.3
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
25.6
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
Other publictions for EC 3.1.3.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749824
Yang
Structural and biochemical ch ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
501
674-681
2018
-
-
1
1
6
-
1
5
-
3
-
-
-
3
-
-
1
-
-
-
-
-
20
1
-
-
-
5
-
-
-
-
-
-
-
-
-
2
-
1
6
-
-
2
-
5
-
6
-
-
-
-
-
2
-
-
-
-
20
2
-
-
-
5
-
-
-
-
-
-
-
-
5
5
750203
Terakawa
Bacillus subtilis 5'-nucleoti ...
no activity in Bacillus subtilis
BMC Microbiol.
16
1-13
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
-
-
-
-
-
722956
Zimmerman
Structural insight into the me ...
Escherichia coli
J. Mol. Biol.
378
215-226
2008
-
-
1
1
10
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
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-
1
-
1
10
-
-
-
-
4
-
-
-
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-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
656266
Proudfoot
General Enzymatic Screens Iden ...
Escherichia coli
J. Biol. Chem.
279
54687-54694
2004
-
-
-
-
-
-
11
7
-
3
3
-
-
1
-
-
-
-
-
-
-
-
8
2
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
7
-
3
3
-
-
-
-
-
-
-
-
-
8
2
-
-
-
7
-
1
-
-
-
-
-
-
7
7