Literature summary for 3.1.3.86 extracted from

Le Coq, J.; Heredia Gallego, L.; Lietha, D.
Expression, purification, crystallisation and X-ray crystallographic analysis of a truncated form of human Src homology 2 containing inositol 5-phosphatase 2 (2016), Protein J., 35, 225-230 .

Search for more literature for 3.1.3.86

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
a fragment of human SHIP2 containing the phosphatase domain and a region proposed to resemble a C2 domain is crystallized by the hanging drop vapor diffusion method, using 0.1 M Bis-Tris propane pH 7.0, 0.2 M NaNO3, 20% (w/v) PEG 3350, 0.025 % (w/v) CH2Cl2, 2 mM Tris(2-carboxyethyl)phosphine	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O	Homo sapiens	-	1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O15357	-	-

Purification (Commentary)

Purification (Comment)	Organism
GST resin column chromatography, Source 15S column chromatography, and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O	-	Homo sapiens	1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 500005, a fragment of human SHIP2 containing the phosphatase domain and a region proposed to resemble a C2 domain, SDS-PAGE	Homo sapiens
?	x * 52685, a fragment of human SHIP2 containing the phosphatase domain and a region proposed to resemble a C2 domain, electrospray ionization mass spectrometry	Homo sapiens
?	x * 52688, a fragment of human SHIP2 containing the phosphatase domain and a region proposed to resemble a C2 domain, calculated from amino acid sequence	Homo sapiens

Synonyms

Synonyms	Comment	Organism
INPPL1	-	Homo sapiens
SHIP2	-	Homo sapiens
Src homology 2 containing inositol 5-phosphatase 2	-	Homo sapiens

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Release 2023.1 (January 2023)