Inhibitors | Comment | Organism | Structure |
---|---|---|---|
inositol hexakissulfate | inhibitory at high concentrations | Rattus norvegicus | |
additional information | N-ethylmaleimide, orthophosphate, 4-nitrophenylphosphate, glycerolbisphosphate, inositol hexakisphosphate and (1R,2R,4R)-cyclohexane-1,2,4-trismethylenesulfonate are without significant effect on the enzyme activity | Rattus norvegicus | |
neomycin | partial inhibition | Rattus norvegicus | |
phosphatidylinositol 4,5-bisphosphate | strong inhibition at low micromolar concentrations | Rattus norvegicus | |
phosphatidylinositol 4-phosphate | inhibition at low micromolar concentrations | Rattus norvegicus | |
vanadate | partial inhibition at millimolar concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
0.1 | - |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | in the presence of 5 mM MgCl2, 0.5 mM EGTA, 50 mM Tris, pH 7.4, temperature not specified in the publication | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Rattus norvegicus | |
additional information | Zn2+, Ca2+ and Mn2+ do not support enzyme activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap S column chromatography, phosphocellulose column chromatography, heparin-HiTrap column chromatography, Q-HiTrap column chromatography, and S-300 gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | - |
Rattus norvegicus | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Rattus norvegicus | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase | - |
Rattus norvegicus |
PtdIns(3,4,5)P3 5-phosphatase | - |
Rattus norvegicus |
SHIP | - |
Rattus norvegicus |