Literature summary for 3.1.3.82 extracted from

Wang, L.; Huang, H.; Nguyen, H.H.; Allen, K.N.; Mariano, P.S.; Dunaway-Mariano, D.
Divergence of biochemical function in the HAD superfamily: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) (2010), Biochemistry, 49, 1072-1081.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli
-	Bordetella bronchiseptica
-	Mesorhizobium loti

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glycero-beta-D-manno-heptose 1-phosphate	noncompetitive inhibitor	Escherichia coli
histidinol	-	Escherichia coli
histidinol phosphate	noncompetitive inhibitor	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
0.0069	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
0.013	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
0.067	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
0.28	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
0.58	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
0.61	-	D-sedoheptulose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
1.5	-	D-fructose 1,6-bisphosphate	pH 7.5, 25°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Bordetella bronchiseptica	Q7WG29	-	-
Escherichia coli	P63228	-	-
Mesorhizobium loti	Q98I56	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
-	Bordetella bronchiseptica
-	Mesorhizobium loti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	poor substrate	Escherichia coli	D-fructose 1-phosphate + phosphate	-	?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer	Escherichia coli	D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate	-	?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer	Bordetella bronchiseptica	D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate	-	?
D-glycero-alpha-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer	Mesorhizobium loti	D-glycero-alpha-D-manno-heptose 1-phosphate + phosphate	-	?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 100fold higher than for the alpha-anomer	Escherichia coli	D-glycero-beta-D-manno-heptose 1-phosphate + phosphate	-	?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 150fold higher than for the alpha-anomer	Bordetella bronchiseptica	D-glycero-beta-D-manno-heptose 1-phosphate + phosphate	-	?
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O	kcat/Km for the beta-anomer is 180fold higher than for the alpha-anomer	Mesorhizobium loti	D-glycero-beta-D-manno-heptose 1-phosphate + phosphate	-	?
D-sedoheptulose 1,7-bisphosphate + H2O	-	Escherichia coli	?	-	?
additional information	no activity with: fructose 6-phosphate, fructose 1-phosphate, alpha-D-glucose 1,6-bisphosphate, beta-D-glucose 1,6-bisphosphate, alpha-D-mannose 1,6-bisphosphate, and beta-D-mannose 1,6-bisphosphate, histidinol	Escherichia coli	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.039	-	D-fructose 1,6-bisphosphate	pH 7.5, 25°C	Escherichia coli
0.51	-	D-sedoheptulose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
4.4	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
4.6	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
5.9	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
18	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
22	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
35.7	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.06	-	D-glycero-beta-D-manno-heptose 1-phosphate	pH 7.5, 25°C, Ki(slope)	Escherichia coli
0.15	-	D-glycero-beta-D-manno-heptose 1-phosphate	pH 7.5, 25°C, Ki(intercept)	Escherichia coli
1	-	histidinol	pH 7.5, 25°C, Ki(slope)	Escherichia coli
6	-	histidinol	pH 7.5, 25°C, Ki(intercept)	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.026	-	D-fructose 1,6-bisphosphate	pH 7.5, 25°C	Escherichia coli
0.84	-	D-sedoheptulose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
7.6	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
21	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
69	-	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli
1385	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Mesorhizobium loti
3188	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Bordetella bronchiseptica
7140	-	D-glycero-beta-D-manno-heptose 1,7-bisphosphate	pH 7.5, 25°C	Escherichia coli

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Release 2023.1 (January 2023)