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Literature summary for 3.1.3.8 extracted from

  • Shim, J.H.; Oh, B.C.
    Characterization and application of calcium-dependent ?-propeller phytase from Bacillus amyloliquefaciens DS11 (2012), J. Agric. Food Chem., 60, 7532-7537.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D308A 50% of wild-type activity, significant decrease in melting temperature and optimum temperature Bacillus sp. (in: Bacteria)
D308E 94% of wild-type activity, significant decrease in melting temperature and optimum temperature Bacillus sp. (in: Bacteria)
D56A 0.4% of wild-type activity, significant decrease in melting temperature and optimum temperature Bacillus sp. (in: Bacteria)
D56E 20% of wild-type activity, significant decrease in melting temperature and optimum temperature Bacillus sp. (in: Bacteria)
D56L 0.6% of wild-type activity, significant decrease in melting temperature and optimum temperature Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1363
-
myo-inositol hexakisphosphate wild-type, pH 9.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.138
-
myo-inositol hexakisphosphate wild-type, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.143
-
myo-inositol hexakisphosphate wild-type, pH 8.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.146
-
myo-inositol hexakisphosphate wild-type, pH 6.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.151
-
myo-inositol hexakisphosphate wild-type, pH 5.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.156
-
myo-inositol hexakisphosphate mutant D308E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.159
-
myo-inositol hexakisphosphate wild-type, pH 4.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.167
-
myo-inositol hexakisphosphate mutant D308A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.189
-
myo-inositol hexakisphosphate mutant D56E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.218
-
myo-inositol hexakisphosphate mutant D56L, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.248
-
myo-inositol hexakisphosphate mutant D56A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme requires excess Ca2+ for its enzymatic activity as the pH decreases Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) O66037
-
-
Bacillus sp. (in: Bacteria) DS11 O66037
-
-

Renatured (Commentary)

Renatured (Comment) Organism
enzyme can restore its enzymatic activity in the presence of 5 mM Ca2+ to 87% of its original activity after heating to 100°C and subsequent cooling Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Bacillus sp. (in: Bacteria) myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Bacillus sp. (in: Bacteria) DS11 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
beta-propeller phytase
-
Bacillus sp. (in: Bacteria)
BPP
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
mutants D56L, D308A Bacillus sp. (in: Bacteria)
55
-
mutant D308E Bacillus sp. (in: Bacteria)
60
-
mutant D56E Bacillus sp. (in: Bacteria)
80
-
wild-type Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.6
-
mutant D308A, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)
48.8
-
wild-type, melting temperature, presence of EDTA, mutant D56A, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)
49.6
-
mutant D56L, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)
54.8
-
mutant D308E, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)
55.5
-
mutant D56E, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)
80
-
wild-type, melting temperature, presence of 5 mM Ca2+ Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
myo-inositol hexakisphosphate mutant D56A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.1
-
myo-inositol hexakisphosphate mutant D56L, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
3.34
-
myo-inositol hexakisphosphate mutant D56E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
8.33
-
myo-inositol hexakisphosphate mutant D308A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
15.55
-
myo-inositol hexakisphosphate mutant D308E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
16.55
-
myo-inositol hexakisphosphate wild-type, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.5
-
Ca2+ wild-type, pH 9.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
5.2
-
Ca2+ wild-type, pH 8.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
7.4
-
Ca2+ wild-type, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
17.6
-
Ca2+ wild-type, pH 6.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
43.6
-
Ca2+ wild-type, pH 5.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
738
-
Ca2+ wild-type, pH 4.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.27
-
myo-inositol hexakisphosphate mutant D56A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
0.44
-
myo-inositol hexakisphosphate mutant D56E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
17.67
-
myo-inositol hexakisphosphate mutant D56L, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
49.9
-
myo-inositol hexakisphosphate mutant D308A, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
99.7
-
myo-inositol hexakisphosphate mutant D308E, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)
119.9
-
myo-inositol hexakisphosphate wild-type, pH 7.0, temperature not specified in the publication Bacillus sp. (in: Bacteria)