Protein Variants | Comment | Organism |
---|---|---|
D308A | 50% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
D308E | 94% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
D56A | 0.4% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
D56E | 20% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
D56L | 0.6% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1363 | - |
myo-inositol hexakisphosphate | wild-type, pH 9.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.138 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.143 | - |
myo-inositol hexakisphosphate | wild-type, pH 8.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.146 | - |
myo-inositol hexakisphosphate | wild-type, pH 6.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.151 | - |
myo-inositol hexakisphosphate | wild-type, pH 5.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.156 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.159 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.167 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.189 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.218 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.248 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme requires excess Ca2+ for its enzymatic activity as the pH decreases | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | O66037 | - |
- |
Bacillus sp. (in: Bacteria) DS11 | O66037 | - |
- |
Renatured (Comment) | Organism |
---|---|
enzyme can restore its enzymatic activity in the presence of 5 mM Ca2+ to 87% of its original activity after heating to 100°C and subsequent cooling | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) DS11 | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-propeller phytase | - |
Bacillus sp. (in: Bacteria) |
BPP | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
mutants D56L, D308A | Bacillus sp. (in: Bacteria) |
55 | - |
mutant D308E | Bacillus sp. (in: Bacteria) |
60 | - |
mutant D56E | Bacillus sp. (in: Bacteria) |
80 | - |
wild-type | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48.6 | - |
mutant D308A, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
48.8 | - |
wild-type, melting temperature, presence of EDTA, mutant D56A, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
49.6 | - |
mutant D56L, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
54.8 | - |
mutant D308E, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
55.5 | - |
mutant D56E, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
80 | - |
wild-type, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.1 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
3.34 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
8.33 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
15.55 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
16.55 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
Ca2+ | wild-type, pH 9.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
5.2 | - |
Ca2+ | wild-type, pH 8.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
7.4 | - |
Ca2+ | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
17.6 | - |
Ca2+ | wild-type, pH 6.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
43.6 | - |
Ca2+ | wild-type, pH 5.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
738 | - |
Ca2+ | wild-type, pH 4.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
0.44 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
17.67 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
49.9 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
99.7 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
119.9 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |