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Literature summary for 3.1.3.8 extracted from

  • Huang, H.; Zhang, R.; Fu, D.; Luo, J.; Li, Z.; Luo, H.; Shi, P.; Yang, P.; Diao, Q.; Yao, B.
    Diversity, abundance and characterization of ruminal cysteine phytases suggest their important role in phytate degradation (2011), Environ. Microbiol., 13, 747-757.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis Bos taurus
genes phyG1 and phyG51, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis Capra hircus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted phytases PhyG1 and PhyG51 Capra hircus
-
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Capra hircus
-
genes phyG1 and phyG51
-

Source Tissue

Source Tissue Comment Organism Textmining

Synonyms

Synonyms Comment Organism
cysteine phytase
-
Bos taurus
cysteine phytase
-
Capra hircus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bos taurus
50
-
-
Capra hircus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 80% of maximal activity at 39°C, maximal activity at 50°C Bos taurus
37 50 80% of maximal activity at 39°C, maximal activity at 50°C Capra hircus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
isozyme r-PhyG1 loses over 80% of its activity after incubation at 50°C for 15 min, whereas isozyme r-phyG51 is not affected by these conditions Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
secreted isozyme PhyG1 Capra hircus
6.5
-
secreted isozyme PhyG51 Capra hircus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 Bos taurus
5 7 the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 Capra hircus

General Information

General Information Comment Organism
physiological function cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment Bos taurus
physiological function cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment Capra hircus