Literature summary for 3.1.3.8 extracted from

Oakley, A.J.
The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic (2010), Biochem. Biophys. Res. Commun., 397, 745-749.

Crystallization (Commentary)

Crystallization (Comment)	Organism
deglycosylated PhyA free or in complex with inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, vapour-diffusion hanging drop technique, reservoirs contain 1 ml of PEG 3350 at 15-30% w/v, and ammonium nitrate a t0.1-0.4 M, siliconized coverslips are used to suspend a drop containing 0.002 ml of phytase at 13 mg/ml protein concetration and 0.002 ml of reservoir solution over the reservoir, X-ray diffraction structure determination and analysis at 2.2-2.4 A resolution	Aspergillus niger

Crystallization (Comment)

Organism

deglycosylated PhyA free or in complex with inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, vapour-diffusion hanging drop technique, reservoirs contain 1 ml of PEG 3350 at 15-30% w/v, and ammonium nitrate a t0.1-0.4 M, siliconized coverslips are used to suspend a drop containing 0.002 ml of phytase at 13 mg/ml protein concetration and 0.002 ml of reservoir solution over the reservoir, X-ray diffraction structure determination and analysis at 2.2-2.4 A resolution

Aspergillus niger

Inhibitors

Inhibitors	Comment	Organism	Structure
myo-inositol-1,2,3,4,5,6-hexakissulfate	potent inhibition, the compound is isosteric and isoelectronic with respect to substrate myo-inositol-1,2,3,4,5,6-hexakisphosphate	Aspergillus niger

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus niger	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE	Aspergillus niger
60000	-	x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE	Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	Aspergillus niger	-	? + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	gene phyA	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Aspergillus niger	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O	-	Aspergillus niger	? + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, glycosylated enzyme, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE	Aspergillus niger

Synonyms

Synonyms	Comment	Organism
myo-inositol-hexaphosphate phosphohydrolase	-	Aspergillus niger
PhyA	-	Aspergillus niger

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	5.5	-	Aspergillus niger

pH Range

pH Minimum	pH Maximum	Comment	Organism
2.5	5.5	pH-activity profile, with optima at pH 2.5 with 60% of maximal activity, and at pH 5.0-5.5 with maximal activity, and a local minimum at pH 3.5	Aspergillus niger

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0046	-	myo-inositol-1,2,3,4,5,6-hexakissulfate	pH 5.0-5.5, temperature not specified in the publication	Aspergillus niger