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Literature summary for 3.1.3.8 extracted from
- Gene cloning and characterization of a thermostable phytase from Bacillus subtilis US417 and assessment of its potential as a feed additive in comparison with a commercial enzyme (2008), Mol. Biotechnol., 40, 127-135.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, complete inhibition | Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
myo-inositol hexakisphosphate | pH 7.5, 55°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41800 | - |
x * 41800, calculated | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | Q84B22 | - |
- |
| Bacillus subtilis US417 | Q84B22 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 7.5, 55°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | 80% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis | ? + phosphate | - |
? | |
| ADP + H2O | 80% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis US417 | ? + phosphate | - |
? | |
| AMP + H2O | 10% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis | adenosine + phosphate | - |
? | |
| AMP + H2O | 10% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis US417 | adenosine + phosphate | - |
? | |
| ATP + H2O | 97% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis | ? + phosphate | - |
? | |
| ATP + H2O | 97% of the activity with myo-inositol hexakisphosphate | Bacillus subtilis US417 | ? + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis US417 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41800, calculated | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 22% of initial activity, absence of Ca2+ | Bacillus subtilis |
| 75 | - |
10 min, 77% of initial activity, presence of 5 mM Ca2+ | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
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