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Literature summary for 3.1.3.8 extracted from
- Screening, cloning and overexpression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics (2007), Lett. Appl. Microbiol., 45, 522-528.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.196 | - |
myo-inositol hexakisphosphate | in 1 M sodium acetate buffer pH 5.5, at 37°C | Aspergillus niger |  |
| 18.16 | - |
p-nitrophenyl phosphate | in 1 M sodium acetate buffer pH 5.5, at 37°C | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49200 | - |
calculated molecular weight | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | Q6T9Z6 | - |
- |
| Aspergillus niger N-J | Q6T9Z6 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 503 | - |
recombinant enzyme, at 37°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger N-J | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Aspergillus niger | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Aspergillus niger N-J | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| myo-inositol hexaphosphate phosphohydrolase | - |
Aspergillus niger |
| PhyA | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant enzyme, with 10 min reaction time | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
retains 70% activity after exposure to 90°C for 5 min and 65% activity after 30 min | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
p-nitrophenyl phosphate | in 1 M sodium acetate buffer pH 5.5, at 37°C | Aspergillus niger | |
| 398 | - |
myo-inositol hexakisphosphate | in 1 M sodium acetate buffer pH 5.5, at 37°C | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
recombinant enzyme | Aspergillus niger |
| 5.5 | - |
recombinant enzyme | Aspergillus niger |
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