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Literature summary for 3.1.3.77 extracted from
- Purification, crystallization and preliminary X-ray diffraction analysis of human enolase-phosphatase E1 (2005), Acta Crystallogr. Sect. F, 61, 521-523.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the bacterial expression vector pET28a with a His tag on the C-terminus. Recombinant plasmid overexpressed in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| by hanging drop vapour-diffusion method, to 1.7 A resolution, crystals belong to space group P212121, with unit-cell parameters a = 54.02 A, b = 57.55 A, c = 87.32 A | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+-chelating affinity column, gel filtration and anion-exchange chromatography, more than 90% pure | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enolase-phosphatase | - |
Homo sapiens |
| MASA | - |
Homo sapiens |
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