KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
2-hydroxy-3-keto-1-phospho-1-hexene | - |
Klebsiella pneumoniae | |
0.077 | - |
2,3-Diketo-1-phosphohexane | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
gel filtration | Klebsiella pneumoniae |
27000 | - |
calculated from sequence | Klebsiella pneumoniae |
28300 | - |
SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
CG253 | - |
Klebsiella pneumoniae CG253 | - |
CG253 | - |
Purification (Comment) | Organism |
---|---|
100fold purified to homogeneity in 10% yield, SDS-PAGE, amino acid sequence analysis | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
purified enzyme by phenyl-Superose column | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-diketo-1-phosphohexane + H2O | including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene | Klebsiella pneumoniae | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
2,3-diketo-1-phosphohexane + H2O | including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene | Klebsiella pneumoniae CG253 | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
2-hydroxy-3-keto-1-phospho-1-hexene + H2O | - |
Klebsiella pneumoniae | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? | |
2-hydroxy-3-keto-1-phospho-1-hexene + H2O | - |
Klebsiella pneumoniae CG253 | 2-ketopentanoate + formate + glyoxylate + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
E-1 | - |
Klebsiella pneumoniae |