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Literature summary for 3.1.3.75 extracted from

  • May, A.; Spinka, M.; Koeck, M.
    Arabidopsis thaliana PECP1 - Enzymatic characterization and structural organization of the first plant phosphoethanolamine/phosphocholine phosphatase (2012), Biochim. Biophys. Acta, 1824, 319-325.
    View publication on PubMed
Search for more literature for 3.1.3.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
1.16
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
Fe2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
Mg2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana
additional information not activated by Ca2+, Zn2+, or Cu2+ Arabidopsis thaliana
Ni2+ divalent metal ions required, highest activity with 1.5 mM Mg2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31570
-
 calculated from amino acid sequence Arabidopsis thaliana
32000
-
 gel filtration, SDS-PAGE Arabidopsis thaliana
64000
-
 native gel elelctrophoresis, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphocholine + H2O Arabidopsis thaliana
-
 choline + phosphate
-
 ?
phosphoethanolamine + H2O Arabidopsis thaliana
-
 ethanolamine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FZ62
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose activated column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
 substrate phosphocholine, 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
3.67
-
 substrate phosphoethanolamine, 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-80°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are 4-nitrophenyl phosphate, diphosphate, phospho-L-serine, phospho-L-tyrosine Arabidopsis thaliana ?
-
 ?
phosphocholine + H2O
-
 Arabidopsis thaliana choline + phosphate
-
 ?
phosphoethanolamine + H2O
-
 Arabidopsis thaliana ethanolamine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active Arabidopsis thaliana
monomer 1 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtPECP1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.04
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
13
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 7.8
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana theoretical value
-
 5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.06
-
 Phosphoethanolamine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana
2.34
-
 phosphocholine 50 mM Hepes/NaOH buffer, pH 7.0, 37°C Arabidopsis thaliana