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Literature summary for 3.1.3.75 extracted from
- Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization (2007), J. Bone Miner. Res., 22, 617-627.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PHOSPHO1 expression is not influenced by presence of tissue-nonspecific alkaline phosphatase | Mus musculus |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | PHOSPHO1 plays a role in the initiation of matrix mineralization | Gallus gallus |
| medicine | PHOSPHO1 plays a role in the initiation of matrix mineralization | Mus musculus |
| medicine | PHOSPHO1 plays a role in the initiation of matrix mineralization | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into vector pBAD TOPO TA, expressed in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-phenyl-1,2-benzoisoselenazol-3(2H)-one | noncompetitive inhibitor | Homo sapiens |  |
| ebselen | - |
Homo sapiens | |
| lansoprazole | 1 mM decreases calcifying potential in the presence of phosphoethanolamine by 10% | Gallus gallus | |
| lansoprazole | noncompetitive inhibitor, reduces activity by 28% | Homo sapiens | |
| lansoprazole | inhibits the mineralization of matrix vesicles from tissue-nonspecific alkaline phosphatase deficient Akp2(-/-) osteoblasts by 56.8% | Mus musculus | |
| SCH202676 | 1 mM decreases calcifying potential in the presence of phosphoethanolamine by 10% | Gallus gallus | |
| SCH202676 | noncompetitive inhibitor, reduces activity by 16% | Homo sapiens | |
| SCH202676 | inhibits the mineralization of matrix vesicles from tissue-nonspecific alkaline phosphatase deficient Akp2(-/-) osteoblasts by 70.7% | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Gallus gallus | |
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
PHOSPHO1 splice variant, Western blot analysis | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | low expression, 3.38fold higher than in liver | Mus musculus | - |
| bone | matrix vesicles | Gallus gallus | - |
| bone | PHOSPHO1 is restricted to mineralizing regions of bone and growth plate. 120fold higher level of PHOSPHO1 expression in bone compared with liver. Enzyme present within matrix vesicles is in an active state. Present in early hypertrophic chondrocytes of the growth plate, and in osteoblasts of trabecular surfaces and infilling primary osteons of cortical bone | Mus musculus | - |
| brain | low expression, 1.96fold higher than in liver | Mus musculus | - |
| gut | low expression, 1.2fold higher than in liver | Mus musculus | - |
| heart | low expression, 5.67fold higher than in liver | Mus musculus | - |
| liver | lowest expression | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phosphoethanolamine + H2O | - |
Gallus gallus | ethanolamine + phosphate | - |
? | |
| O-phosphoethanolamine + H2O | - |
Mus musculus | ethanolamine + phosphate | - |
? | |
| O-phosphoethanolamine + H2O | - |
Homo sapiens | ethanolamine + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHOSPHO1 | - |
Gallus gallus |
| PHOSPHO1 | - |
Mus musculus |
| PHOSPHO1 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
- |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00108 | - |
SCH202676 | - |
Homo sapiens | |
| 0.031 | - |
ebselen | - |
Homo sapiens | |
| 0.07128 | - |
lansoprazole | - |
Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00197 | - |
- |
Homo sapiens | SCH202676 | |
| 0.00281 | - |
- |
Homo sapiens | ebselen | |
| 0.00471 | - |
- |
Homo sapiens | lansoprazole | |
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