Literature summary for 3.1.3.75 extracted from

Roberts, S.J.; Stewart, A.J.; Schmid, R.; Blindauer, C.A.; Bond, S.R.; Sadler, P.J.; Farquharson, C.
Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2 (2005), Biochim. Biophys. Acta, 1752, 73-82.

Search for more literature for 3.1.3.75

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and mutant fragment comprising residues M19-C267 in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D123N	site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment	Homo sapiens
D203S	site-directed mutagenesis, inactive mutant enzyme fragment	Homo sapiens
D32N	site-directed mutagenesis, inactive mutant enzyme fragment	Homo sapiens
D43N	site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment	Homo sapiens
D43N/D123N	site-directed mutagenesis, inactive mutant enzyme fragment	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
O-phosphocholine + H2O	Homo sapiens	-	choline + phosphate	-	?
O-phosphoethanolamine + H2O	Homo sapiens	-	ethanolamine + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8TCT1	PHOSPHO1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant fragments M19-C267 from Escherichia coli by nickel affinity chromatography to homogeneity	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
O-phosphoethanolamine + H2O = ethanolamine + phosphate	residues D32 and D203 of catalytic motifs are essential for catalytic activity, residues D43 and D123 are important for substrate binding and specificity	Homo sapiens	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.092	-	purified recombinant mutant D43N enzyme fragment M19-C267, substrate O-phosphoethanolamine	Homo sapiens
0.28	-	purified recombinant mutant D123N enzyme fragment M19-C267, substrate O-phosphoethanolamine	Homo sapiens
4.96	-	purified recombinant wild-type enzyme fragment M19-C267, substrate O-phosphoethanolamine	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	high substrate specificity of PHOSPHO1, residual activity with beta-glycerol phosphate, 4-nitrophenyl phosphate, and ribose 5-phosphate, no activity with phospho-L-serine, diphosphate, fructose 6-phosphate, phospho-L-tyrosine, and ATP, overview	Homo sapiens	?	-	?
O-phosphocholine + H2O	-	Homo sapiens	choline + phosphate	-	?
O-phosphoethanolamine + H2O	-	Homo sapiens	ethanolamine + phosphate	-	?

Subunits

Subunits	Comment	Organism
More	structure motif analysis using the amino acid sequence structural modelling and ligand docking	Homo sapiens

Synonyms

Synonyms	Comment	Organism
More	PHOSPHO1 belongs to the haloacid dehalogenase superfamily	Homo sapiens
PHOSPHO1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.7	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)