Application | Comment | Organism |
---|---|---|
drug development | transduction of the PEP-1-PLPP fusion protein into cells would be useful as therapeutic agent for various disorders related to this enzyme and vitamin B6 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
PLPP gene fused with a PEP-1 peptide (PEP-1-PLPP) and 6His-tag and cloned into pET-15b. Expression in Escherichia coli BL21 (DE3) transduced into PC12 cells at various times and concentrations to evaluate the transduction ability (analyzed by Western blotting, fluorescence labeled). PLP concentration in cells PC12 cells changed by transduced PEP-1-PLPP fusion protein (spectroscopic measurement) | Homo sapiens |
General Stability | Organism |
---|---|
recombinant PEP-1-PLPP fusion protein transduced into PC12 cells is stable for 36 h | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE, Western blot (rabbit anti-histidine polyclonal antibody) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxal 5'phosphate + H2O | Homo sapiens | - |
pyridoxal + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96GD0 | - |
- |
Purification (Comment) | Organism |
---|---|
sonication of cells in binding buffer (5 mM imidazole, 0.5 M NaCl, 20 mM Tris-HCl, pH 7.9), centrifugation, Ni2+-nitrilotriacetic acid Sepharose affinity column, salt removed with PD10 column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
crude extract contains 0.13 units/mg, purified PEP-1-PLPP fusion protein contains 1.5 units/mg, after purification of recombinant enzyme, pH 7.4 in 40 mM triethanolamineamine-HCl, measuring absorbance reduction at 390 nm (3 min) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxal 5'phosphate + H2O | - |
Homo sapiens | pyridoxal + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyridoxal-5'-phosphate phosphatase | PLPP | Homo sapiens |